6C-kine or Exodus2 is a novel CC chemokine discovered independently by three groups from the EST database. 6C-kine, also named SLC (Secondary Lymphoidtissue Chemokine), and CCL21, shows 21 33% identity to other CC chemokines. 6C-kine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxyl-terminal domain. Human 6C-kine cDNA encodes a 134 amino acid residue, highly basic, precursor protein with a 23 amino acid residue signal peptide that is cleaved to form the predicted 111 amino acid residue mature protein. Mouse 6C-kine cDNA encodes a 133 amino acid residue protein with a 23 residue signal peptide that is cleaved to generate the 110 residue mature protein. Human and mouse 6C-kine are highly conserved, exhibiting 86% amino acid sequence identity. 6Ckine is constitutively expressed at high levels in lymphoid tissues such as lymph nodes, spleen and appendix. In mouse, high levels of 6Ckine mRNA are also detected in the lung. The gene for human 6C-kine has been localized at human chromosome 9p13 rather than chromosome 17 where the genes of many human CC chemokines are clustered. The 6C-kine gene location is within a region of about 100 kb from the gene for MIP3β/ ELC, another novel CC chemokine. Unlike most CC chemokines, 6C-kine is not chemotactic for monocytes. Recombinant mouse 6Ckine is chemotactic in vitro for thymocytes and activated T cells. Recombinant human 6C-kine has been shown to be chemotactic for some human T cell lines, resting PBL, and cultured T cells expanded with PHA and IL2. 6Ckine has also been reported to inhibit hemopoietic progenitor colony formation in a dosedependent manner. 6C-kine acts via a class of as yet unidentified CC receptors on both T cells and B cells that are not shared by any other CC chemokines so far tested.
1100.74RON
În stoc
Număr Catalog 209-102-P11CategorieAfaceri și industrie > Știință și laboratorFurnizorReliaTechGentaurDimensiune100 µgTipsingle
