Omalizumab (Xolair®) is a recombinant DNA-derived humanized IgG1κ monoclonal antibody that binds human immunoglobulin E (IgE) for the treatment of moderate to severe persistent allergic asthma and Chronic Idiopathic Urticaria (CIU). Omalizumab inhibits the binding of IgE to IgE receptor (FcεRI) on the surface of mast cells and basophils thereby limiting the release of mediators of the allergic response from the FcεRI bearing cells. The Omalizumab ELISA kit is designed to measure free Omalizumab with high specificity and sensitivity . This assay employs the sandwich enzyme immunoassay technique. A precoated anti-Omalizumab 96 well plate is provided. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Omalizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Omalizumab present in test samples and the concentration is calculated from the standard series.
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Număr Catalog 1061-EL-1611-152CategorieAfaceri și industrie > Știință și laboratorFurnizorAffinitylmmunoGentaurDimensiune1X96 wellsTipsingle