The Ubch5b TR-FRET Assay Kit is a homogeneous, sensitive TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) assay kit designed to measure UbcH5b (Ubiquitin-conjugating enzyme E2 D2) ubiquitination activity. It utilizes biotin-labeled Ubiquitin and a Terbium-labeled antibody recognizing the His-tagged Ubch5b protein to complete the TR-FRET pairing. The kit contains enough purified UbcH5b, purified UBE1, Biotin-Ubiquitin, anti-His Tb-labeled donor, dye-labeled acceptor, and assay buffer for 400 reactions.Figure 1: UbcH5b TR-FRET Assay Kit schematic.The Terbium-labeled anti-His antibody binds to the His-tagged E2 conjugating protein, while the Dye-labeled streptavidin acceptor binds to Biotin-Ubiquitin. The complex forms when ubiquitin is transferred to the E2 enzyme, and the TR-FRET signal is measured using a fluorescence plate reader capable of measuring Time Resolved-Fluorescence Resonance Energy Transfer. The TR-FRET signal is proportional to UbcH5b activity.*NOTE: As of January 2024, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.Need us to run inhibitor screens or profile your compounds against UBCH5b? Check out ourUbiquitination Screening Services.
3673.6RON
În stoc
Număr Catalog 421-79896CategorieAfaceri și industrie > Știință și laboratorFurnizorBPS BioscienceGentaurDimensiune384 rxns.Tipsingle
