Ai nevoie de ajutor? [email protected]

product
ADAR1 Dual Luciferase Reporter HEK293 Cell Line
The ADAR1 Luciferase Reporter HEK293 cell line is designed to monitor RNA editing by Adenosine deaminase acting on RNA (ADAR1). This cell line stably expresses ADAR1 under the control of a CMV promoter and a separate ADAR editing reporter construct expressed under the control of another CMV promoter. The reporter contains the gene encoding firefly luciferase, which is constitutively expressed in the cells, upstream of the gene encoding the GluA2 ADAR substrate followed by the Renilla luciferase gene. The sequence corresponding to GluA2 has been modified to contain an amber stop codon (UAG). When edited by ADAR, this stop codon (UAG) will be changed to UIG (A to I edit), which is read as tryptophan (UGG) by the translation machinery. This edit allows translation to occur all the way to the end of the reporter mRNA and results in the expression of Renilla luciferase. Conversely, in the absence of ADAR1 activity, translation terminates at the stop codon and Renilla is not expressed. Reporter activity is read out as the Renilla Luciferase/Firefly luciferase ratio whereby inhibition of ADAR activity, and thus the UAG (stop) to UGG (tryptophan) conversion rate, will result in a dose-dependent decrease in the Renilla luciferase/Firefly luciferase ratio.

0.01 EURAsk for quotation

Indisponibil
Număr Catalog 421-78547
CategorieAfaceri și industrie > Știință și laborator
FurnizorGentaurDimensiune2 vialsTipsingle
Produse asociate
MRE Luciferase Reporter HEK293 Cell Line
519-RC1037
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1864.8 €
NF-kB Luciferase Reporter-HEK293 Cell Line
519-RC1025
1 Vial, Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.
1864.8 €
Human SBE Luciferase Reporter Cell Line-HEK293
870-ABC-RC0121
1 vial
Cere ofertă de preț
TU686 Luciferase Reporter Cell Line
870-ABC-RC735H
each
Cere ofertă de preț
Bloguri asociate
Accidentul vascular cerebral – cele mai importante informații
Postat de

Pe măsură ce înaintăm în vârstă, riscul de a ...

Pepsina – ce este și unde se găsește?
Postat de

În magazinele cu produse naturiste și pe dive...

Te ajutăm să găsești cele mai bune produse pentru cercetarea ta și îți răspundem la întrebări prin chat, email sau telefon. Nu ezita să ne contactezi