The TNKS2 (PARP5B) Chemiluminescent Assay Kit is designed to measure the activity of TNKS2 (Tankyrase 2) for screening and profiling applications. The TNKS2 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant purified TNKS2 enzyme (amino acids 849-1166), reaction substrates, and PARP assay buffer for 100 or 400 enzyme reactions.Figure 1. TNKS2 (PARP5B) Chemiluminescent Assay Kit schematic.Histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+mix (termed PARP Substrate Mixture) is incubated with the TNKS2 enzyme in an optimized assay buffer. The plate is then treated with streptavidin-HRP followed by addition of the ELISA ECL substrate to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to the TNKS2 activity.*NOTE: As of April 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.Need us to run inhibitor screens or profile your compounds against TNKS2 (PARP5B) ? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Services.
2912RON
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Număr Catalog 421-78406CategorieAfaceri și industrie > Știință și laboratorFurnizorBPS BioscienceGentaurDimensiune96 rxns.Tipsingle
